Mission of the Proteomics Core Facility

The Mission of the UNC Proteomics Core Facility is to provide UNC investigators with state-of-the-art scientific and technical support in modern proteomics. This requires a commitment to developing, implementing, and optimizing cutting-edge methodologies and instrumentation. In addition, we believe a strong commitment to educating UNC researchers in the field of proteomics is important.

Center Administrators

Facility Director: Dr. David Smalley (email)

Faculty Director: Dr. Lee Graves (email)

 

General Summary of Service

For full list, click here

1) Identify a purified protein

2) Identify proteins in a complex mixture. For complex mixtures such as a subcellular organelle or a cell lysate, we can identify between 200 and 300 of the most abundant proteins easily. A more in-depth analysis can be performed to identify between 750 and 1500 proteins.

3) Identify differences between 2 (or more) complex protein mixtures. We have the capabilities to utilize both unlabeled and labeled approaches. These include iTRAQ, SILAC, and ICAT. We will assist you with metabolic labeling (e.g. SILAC) but we do not perform this.

4) Identify interacting proteins. See section on co-immunoprecipitations (Link).

5) Identification of Post-Translational Modifications (PTMs) of proteins such as acetylation or phosphorylation.

6) Determination of molecular mass for peptides or proteins.

 

For more specific information, please contact our facility director (contact information above).

 

Sample Submission

Click here

 

Comming Soon:

Online Reservation Calendar for usage of the 4800 MALDI-TOF/TOF (For Trained Users Only.(link)

 

Acknowledgements, Recognition, and Future SerVice

All work provided by the Proteomics Core facility which is included in a publication record should acknowledge the contribution of the core facility. Proper acknowledgment of core facilities enables us to obtain financial and other support so that they may continue to provide their essential services in the best ways possible. It would be appreciated if you sent a copy of the accepted manuscript, published paper, or title of grant application to proteomic@unc.edu. This will allow us to demonstrate our impact the UNC Community.

In addition, if any facility personnel makes a substantial intellectual and/or experimental contribution to a publication they deserve recognition just as any other co-author. This is essential for the professional development of our staff.

We recommend scientist follow the “ABRF Recommended Guidelines for Authorship on Manuscripts”. Simply because services are provided on a Fee-for-service basis doesn’t diminish the importance of our scientists in impacting your research.

 

 

Core Facility of the Lineberger Comprehensive Cancer Center
& CEHS - Systems Biology Facility Core

 

**Note--As you may have noticed, we have recently changed the design of our website. If you have any issues or suggestions, please contact the director.