Methods

Sample Preparation Methods

Protocol 1: Fixing and Staining Gel-separated Proteins (Coomassie):

Gel Fixing and Staining Protocol.pdf

Protocol 2: Sypro Ruby Stain:

Sypro Ruby Stain Protocol.pdf

Protocol 3: Silver Staining (Least compatible with protein ID by MS):

Silverquest Protocol.pdf

Protocol 4: In Solution Tryptic Digest:

Trypsin Digestion Protocol.pdf

Literature Links

http://base-peak.wiley.com/news/glossary.htm - Good source of commony used phrases
and abbreviations in mass spectrometry

http://www.ionsource.com/tutorial/spectut/spec1.htm - Great introduction to interpreting ESI mass
spectra. A must for new ESI users. (The website itself has lots of great reference material.)

http://www.chem.vt.edu/chem-ed/crossref/ac-mass-spec.html - Another good site with more specific instrumentation descriptions

http://welcome.to/gpmaw - GPMAW software site.

Core Facility of the Lineberger Comprehensive Cancer Center
& CEHS - Systems Biology Facility Core