Preferred Staining Methods for 1D and 2D gels:
Protocol
1: Fixing and Staining Gel-separated Proteins (Coomassie):
>
Gel
Fixing and Staining Protocol.pdf
Protocol 2: Sypro Ruby Stain:
> Sypro Ruby Stain Protocol.pdf
Protocol
3: Silver Staining(Least compatible with protein ID by MS):
> Silverquest
Protocol.pdf
Other Methods:
Protocol 4: In-Solution Reduction/Alkylation:
> Reduction-Alkylation
Protocol.pdf
Protocol
5: In Solution Tryptic Digest:
> Trypsin
Digestion Protocol.pdf
Protocol 6: Protocol for the Collection and Storage of Plasma:
> Plasma Protocol.pdf
Protocol 7: Protocol for Collection and Storage of Serum:
> Serum Protocol.pdf
Protocol 8: Depletion Methods for Plasma or Serum Biomarker Studies :
> Depletion Methods Plasma Serum.pdf
NOTE: To improve the chance of success, Invitrogen/Novex Pre-Cast Tris/glycine SDS-PAGE gels and recommended staining procedures MUST be used.
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