Jiang, J., Parker, C.E., Fuller, J.R., Kawulac, T.H., Borchers, C.H., An immunoaffinity tandem mass spectrometry (iMALDI) assay for detection of Francisella tularensis, (2007), Analytica Chimica Acta, in press.
Jiang, J., Parker, C.E., Hoadley, K.A., Perou, C.M.., Boysen, G., Borchers, C.H., Development of an immuno tandem mass spectrometry (iMALDI2) assay for EGFR diagnosis, (2007), J. Proteomics Res., in press.
Myers-Morales,C., Cowan,C., Gray, M.E., Wulff, C.R.1, Parker, C., Borchers, C., Straley, S.C. A surface-focused biotinylation procedure identifies the Yersinia pestis catalase KatY as a membrane-associated but non-surface-located protein, Applied and Environmental Microbiology (2007), 73(18), 5750-5759.
Vemuganti, S.A, Bell,T.A., Scarlett, C.O., Parker, C.E., Pardo-Manuel de Villena, F. O’Brien, D.A. Three male germline-specific aldolase A isozymes are generated by alternative splicing and retrotransposition, (2007), Developmental Biology (San Diego, CA, United States) (2007), 309(1), 18-31.
Mahajan, N.P., Liu, Y., Majumder, S., Warren, M.W., Parker, C. E., Mohler, J.L., Earp, H.S., Whang, Y.E. Activated cdc42-associated kinase Ack1 promotes prostate cancer progression via androgen receptor tyrosine phosphorylation, PNAS (2007), 104(20), 8438-8443.
Reid, J.D., Parker, C.E., Borchers, C.H. Protein arrays for biomarker discovery, (2007) Current Opinion in Molecular Therapeutics 9(3), 216-221.
Maslov, D.A., Spremulli, L. L.; Sharma, M. R.; Bhargava, K., Grasso, D., Falick, A. M., Agrawal, R. K., Parker, C. E., Simpson, L. Proteomics and electron microscopic characterization of the unusual mitochondrial ribosome-related 45S complex in Leishmania tarentolae. Molecular & Biochemical Parasitology (2007), 152(2), 203-212.
Whalen, E.J.; Foster, M. W.; Matsumoto, A.; Ozawa, K., Violin, Jonathan D.; Que, Loretta G.; Nelson, Chris D.; Benhar, M.; Keys, J. R.; Rockman, H. A.; Koch, W. J.; Daaka, Y., Lefkowitz, Robert J.; Stamler, Jonathan S.. Regulation of b-adrenergic receptor signaling by S-nitrosylation of G-protein-coupled receptor kinase 2. Cell (Cambridge, MA, United States) (2007), 129(3), 511-522.
Boysen, G.; Scarlett, C. O.; Temple, B.; Combs, T. P.; Brooks, N. L.; Borchers, C. H.; Swenberg, J. A. Identification of covalent modifications in P450 2E1 by 1,2-epoxy-3-butene in vitro. Chemico-Biological Interactions (2007), 166(1-3), 170-175.
Thelin, W. R.; Chen, Yun; Gentzsch, M.; Kreda, S. M.; Sallee, J. L.; Scarlett, C. O.; Borchers, C. H.; Jacobson, K.; Stutts, M. J.; Milgram, S. L. Direct interaction with filamins modulates the stability and plasma membrane expression of CFTR. Journal of Clinical Investigation (2007), 117(2), 364-374.
2006
Sauls, D.L., Lockhart, E., Warren, M. E., Lenkowski, A., Wilhelm, S.E., and Hoffman, M. Modification of Fibrinogen by Homocysteine Thiolactone Increases Resistance to Fibrinolysis: A Potential Mechanism of the Thrombotic Tendency in Hyperhomocysteinemia. Biochemistry. 2006 Feb 28;45(8):2480-2487.
Tsukada Y, Fang J, Erdjument-Bromage H, Warren M. E., Borchers C. H., Tempst P, and Zhang Y. Histone demethylation by a family of JmjC domain-containing proteins.
Nature. 2006 Feb 16;439(7078):811-6.
Heo, J.; Raines, K. W.; Mocanu, V.; Campbell, S. L. Redox Regulation of RhoA. Biochemistry (2006), 45(48), 14481-14489.
DeLuca, Jennifer G.; Gall, Walter E.; Ciferri, Claudio; Cimini, Daniela; Musacchio, Andrea; Salmon, E. D. Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell (Cambridge, MA, United States) (2006), 127(5), 969-982.
Robinette, David; Neamati, Nouri; Tomer, Kenneth B.; Borchers, Christoph H. Photoaffinity labeling combined with mass spectrometric approaches as a tool for structural proteomics. Expert Review of Proteomics (2006), 3(4), 399-408.
Borchers, Christoph; Chen, Ting; Neamati, Nouri. Application of proteomics in basic biological sciences and cancer. Molecular Carcinogenesis and the Molecular Biology of Human Cancer (2006), 263-288.
2005
Heo J., Prutzman K. C., Mocanu V., and Campbell S. L. Mechanism of Free Radical Nitric Oxide-mediated Ras Guanine Nucleotide Dissociation. Journal of Molecular Biology (2005), 346(5), 1423-1440.
Parker C.E., Warren M.R., Loiselle D.R., Dicheva N.N., Scarlett C.O., Borchers C.H., Identification of Components of Protein Complexes, in Methods in Molecular Biology (Patterson, W. C., ed.), Humana Press, Totowa, NJ (2005), 301, 117-51.
Parker C.E., Mocanu V., Warren M.R.E., Greer S.F., Borchers C.H., Mass Spectrometric Determination of Protein Ubiquitination, in Methods in Molecular Biology (Patterson, W. C., ed.), Humana Press, Totowa, NJ (2005), 301, 153-173.
Warren, M.R.E., Parker, C.E., Mocanu, V., Klapper, D., Borchers, C.H., ESI-MS/MS of Model Peptides Reveals Diagnostic Fragment Ions for Protein Ubiquitination, Rapid Communications in Mass Spectrometry (2005), 19, 1-9.
Murphy M.M., Zayed M.A., Evans A., Parker C.E., Ataga K.I., Telen M.J., Parise, L.V. Role of Rap1 in promoting sickle red blood cell adhesion to laminin via BCAM/LU, Blood, 2005, 105(8), 3322-3329.
Loiselle, D.R., Thelin, W.R., Parker, C.E., Dicheva, N.N., Kesner, B.A., Mocanu, V., Wang, F., Milgram, S.L., Warren, M.R.E., Borchers C.H., Improved Protein Identification through the Use of Unstained Gels, J. Proteome Research, (2005), 4(3), 992-997.
Parnell S.C., Marotti L.A., Jr., Kiang L., Torres M., Borchers C., and Dohlman H., Phosphorylation of the RGS protein Sst2 by the MAP kinase Fus3 and use of Sst2 as a model to analyze determinants of substrate sequence specificity. Biochemistry (2005), 44(22), 8159-8166.
2004
Howell M., Borchers C., and Milgram S.L. Heterogeneous Nuclear Ribonuclear Protein U Associates with YAP and Regulates Its Co-activation of Bax Transcription. JBC (2004), 25, 26300-263006.
Huang, C., Borchers, C., Schaller, M.D., Jacobson, K. Phosphorylation of paxillin by p38mapk is involved in the neurite outgrowth of PC-12 cells. Journal of Cell Biology, (2004), 164(4), 593-602.
Warren, E. N., Elms, P.J., Parker, C.E., Borchers, C.H. Towards the Development of a Protein Chip: An MS-based Method for the Rapid and Sensitive Quantitation of Protein Expression and Modification Levels using a Novel Immunoaffinity Approach, Anal. Chem. (2004), 76(14), 4082-4092.
Hinkle, C. L., Sunnarborg, S. W., Loiselle, D., Parker, C. E., Stevenson, M., Russell, W. E., Lee, D. C. Selective Roles for Tumor Necrosis Factor alpha-converting Enzyme/ADAM17 in the Shedding of the Epidermal Growth Factor Receptor Ligand Family: The Juxtamembrane Stalk Determines Cleavage Efficiency. (2004),J. Biological Chemistry, 279(23), 24179-24188.
Borchers C.H. , Marquez V.E., Schroeder G.K., Short S.A., Snider M.J., Speir P.K., Wolfenden R. Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Reveals the Presence of a Water Molecule in an Enzyme: Transition State Analogue Complex. PNAS, (2004), 101(43), 15341-15345.
Hall M.C., Warren E.N., Borchers C.H. Multi-kinase phosphorylation of the APC/C activator Cdh1 revealed by mass spectrometry. Cell Cycle, (2004), 3 (10), 1278-1284.
2003
Huang C., Rajfur Z., Schaller M., Borchers C., Jacobson K. Phosphorylation of Serine 178 on Paxillin by JNK1 can regulate Cell Migration. Nature (2003), 424(6945), 219-223.
Hall M.C., Torres M.P., Schroeder G., Borchers C.H. Mnd2 and Swm1 are Core Subunits of the Saccharomyces cerevisiae Anaphase-Promoting Complex. Journal of Biological Chemistry (2003), 19, 16698-16705.
Xiao T., Hall H., Kizer K., Sabata Y., Hall M., Borchers C.H., Strahl B.D. Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast. Genes & Dev. (2003), 17, 654
Kast, J., Parker, C.E., van der Drift, K., Dial, J.M., Milgram, S.L., Wilm, M., Howell, M., Borchers, C.H. MALDI-directed nano-ESI-MS/MS Analysis for Protein Identification, Rapid Communications in Mass Spectrometry (2003), 17(16), 1825-1834.
Zheng, L., Dominski, Z., Yang, X-C, Elms, P., Raska C., Borchers C.H., Marzluff W.F. Phosphorylation of SLBP on two threonines triggers degradation of SLBP, the sole cell-cycle regulated factor required for regulation of histone mRNA processing, at the end of S-phase. Mol. Cell. Bio. (2003), 23(5), 1590-1601.
Raska, C.S., Parker, C.E., Sunnarborg, S.W., Pope, R.M., Lee, D.C., Glish, G.L., Borchers, C.H., Rapid and Sensitive Identification of Epitope-Containing Peptides by Direct Matrix Assisted Laser Desorption Ionization Tandem Mass Spectrometry of Peptides Affinity-bound to Antibody Beads, J. Am. Soc. Mass Spectrom., (2003), 14(10). 1076-1085.
Furukawa, M., He, Y.J., Borchers, C., Xiong, Y. Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. Nature Cell Biology (2003), 5(11), 1001-1007.
Zheng, L., Dominski, Z., Yang, X-C, Elms, P., Raska, C., Borchers, C.H., Marzluff W.F. Phosphorylation of SLBP on two threonines triggers degradation of SLBP, the sole cell-cycle regulated factor required for regulation of histone mRNA processing, at the end of S-phase. Mol. Cell. Bio. (2003), 23(5), 1590-1601.
2002
Parker, C. E., Borchers, C. H. Introduction to Proteomics: Tools for the New Biology by Daniel C. Liebler. Chemtracts (2002), 15(13), 747-748.
Dominski, Z., Yang, X.-C., Raska, C. S., Santiago, Carlos, Borchers, C. H., Duronio, R. J., Marzluff, W. F. 3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing system. Molecular and Cellular Biology (2002), 22(18), 6648-6660.
Jun H., Pope, R.M., Borchers, C.H., Graves, L.M., Mapping of Protein Phosphorylation by Dual Enzyme Digestion on MALDI-QoTOF-MS. Anal. Biochem. (2002), 310(2), 214-218.
Liu, J., Shriver, Z., Pope, R. M., Thorp, S. C., Duncan, M.B., Copeland, R. J., Raska, C. S., Yoshida, K., Eisenberg, R. J., Cohen, G., Linhardt, R. J., Sasisekharan, R. Characterization of a Heparan Sulfate Octasaccharide That Binds to Herpes Simplex Virus Type 1 Glycoprotein D. J. Biological Chemistry (2002), 277(36), 33456-33467.
Raska, C.S., Parker, C.E. , Huang, C., Han, J., Glish, G.L., Pope, R.M., Borchers, C.H. Pseudo-MS 3 in a MALDI Orthogonal Quadrupole-time of Flight Mass Spectrometer. J. Am. Soc. Mass Spectrom. (2002), 13:1034-1041.
2001
Pope, R. M ., Raska, C. S., Thorp, S. C., Liu, J. Analysis of heparan sulfate oligosaccharides by nano-electrospray ionization mass spectrometry.Glycobiology (2001), 11(6), 505-513.
Posters Presented at Meetings by Core Personnel
2007
Warren,M.E., Mahajan,N., Parker,C.E., Chen, X., and Earp,H.S. Ack1-mediated Phosphorylation of Androgen Receptor on a Quadrupole Linear Ion Trap and Its Implications to Prostate Cancer, presented at the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-June 7, 2007.
Mocanu, M., Mocanu, V., Dicheva, N.N., Scotchie, J., Fritz, M., Warren, M., Young, S., Parker, C., and Chen, X., Proteomic Analysis of the Human Endometrial Secretome, presented at the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-June 7, 2007.
Wu, Y., Parker, C.E., Warren, M.E., Zhou, L., and Chen, X. Optimization of LC/MS/MS and LC/LC/MS/MS for Plasma and Serum Proteomics, presented at the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-June 7, 2007.
Mocanu, V., Bautz, D., Lionaki, S., Yang, J.J., Hogan, S., Hamra, J., Parker, C.E., Jennette, C.J., Ronald J Falk, R.J., Preston, G.A., and Chen, X. Mass Spectrometry and Autoimmune Disease: Identification and analysis of autoantibodies reactive with human plasma plasminogen, presented at the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-June 7, 2007.
Dicheva, N.N., Parker, C.E., Robinette, D., Mocanu, M., Dai, M., and Chen X. Scanning a Lower Mass Range to Improve Ion Scores and Sequence Coverage for Protein Identification, presented at the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-June 7, 2007.
Parker, C.E., Jing, L., Zhou, L., Warren, M.E., Scarlett, C.O., Danelle, R., and Chen, X., LC- FT- MS Determination of Deamidation, presented at the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-June 7, 2007.
Jing, L., Zhou, L., Warren, M.E., Wu, Y., Dicheva, N.N., Robinette, D., Mocanu, V., Parker, C.E., Seo, D., Ginsburg, G., Schwinn, D., and Chen, X., Optimization of iTRAQ Quantitation for Differential Proteomics in Mice, presented at the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-June 7, 2007.
Jiang, J., Parker, C.E., Hoadley, K.A., Perou, C.M., and Borchers, C.H., Immuno Tandem Mass Spectrometry (iMASS2) Assay for Clinical Proteomics: Development and Application of iMASS2 for EGFR Diagnosis, , presented at the 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 3-June 7, 2007
2006
Jian Jiang; Carol E. Parker; James R. Fuller; Tomas H. Kawula; Christoph H. Borchers, A New Diagnostic Francisella Peptide Chip Assay Allowing Sensitive, Quantitative, and Specific Detection of Francisella in Clinical and Environmental Samples, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Evgeniy V. Petrotchenko; Truc Doant; Christoph H. Borchers, A Novel Chromophoric Affinity-Tagged Isotopically-Coded Crosslinker DGDNBS, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Matthew P. Torres; Jun Han; Hung-Hiang Quek; Craig A. Gelfand; Christoph H. Borchers. Free Flow Electrophoresis (FFE) for MS-based Top-Down Proteomics, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Jian Jiang; Carol E. Parker; Nedyalka N.Dicheva; Christoph H. Borchers, A novel MS- and Affinity-based Peptide (MAP)-Chip for Clinical Cancer Diagnostic, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Jermaine O. Rouson; Cameron O. Scarlett; Bradley Bone; Timothy J. Kirksey; Christoph H. Borchers, A New High Speed Method Of Preparing Reports From MASCOT Search Results, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Evgeniy V. Petrotchenko; Christoph H. Borchers, A Novel Fluorescent Isotopically-Coded MALDI-Cleavable Crosslinker, BiPS, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Kun Lu; Evgeniy V. Petrotchenko; Christoph H. Borchers, Crosslinking and Mass Spectrometry for Identifying Protein-protein Interaction Sites in Activator-Multi-component Protein Complexes, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Jennifer L. Cable; Evgeniy V. Petrotchenko; Nikolay V. Dokholyan; Kunhong Xiao; J. Michael Dial; Robert J. Lefkowitz; Christoph H. Borchers, Detection Of Conformational Changes In Beta-Arrestin By Cross-Linking And Mass Spectrometry, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Cameron O. Scarlett; Ryan M. Danell; Michael Easterling; J. Paul Speir; Christoph H. Borchers, Comparative Analysis of Complex Proteomes for Biomarker Discovery Employing Differential Isotopically-labeled Acetylation Followed by LC/LC-FTMS on a 12 T Instrument, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Severine A. Patat; Brendan McMorran; Cameron O. Scarlett; Claire Wainwright; Brandon Wainwright; Christoph H. Borchers, Top-down Proteomics by 12T-FTICR-MS for Characterizing Protein Biomarkers Discovered by SELDI, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Jongyun Heo; Viorel Mocanu; Kirk C. Prutzman; Sharon L. Campbell; Christoph H. Borchers, Mass Spectrometric Elucidation Of The Free Radical Nitric Oxide-Mediated Ras Guanine Nucleotide Dissociation Mechanism Using Mass Spectrometric Analyses, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Maria E. Warren; Derrick L. Sauls; Carol E. Parker; Maureane Hoffman; Christoph H. Borchers, Homocysteine Thiolactone Modification of Fibrinogen Analyzed by Mass Spectrometry and its Implication to Impaired Fibrinolysis, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Ryan M. Danell; Cameron O. Scarlett; Severine A. Patat; J. Paul Speir; Christoph H. Borchers, Using Data Self-Recalibration and Accurate Mass Fingerprint Searching To Enhance Proteomics Identifications within Complex Mixtures, Presented at the 54th ASMS Conference on Mass Spectrometry and Allied Topics, May 2006, Seattle, WA May 28-June2, 2006.
Boysen, Gunnar; Georgieva, Nadia I.; Scarlett, Cameron O.; Temple, Brenda; Borchers, Christoph H.; Swenberg, James. In vitro evidence for mechanism based inactivation of P450 2E1 by 1,2-epoxy-3-butene. Abstracts of Papers, 232nd ACS National Meeting, San Francisco, CA, United States, Sept. 10-14,2006.
2005
Easterling M. , Speir J.P. , Petrotchenko E. , Marzluff W. , Borchers C.H. , Top-down Proteomics Goes Beyond Bottom-up Proteomics; the Case of the Stem Loop Binding Protein (SLBP), presented at the 53rd ASMS Conference on Mass Spectrometry and Allied Topics, June 2005, San Antonio, TX June 5-9, 2005 .
Gumerov D.R., Scarlett C.O. , Speir J.P., Haney R. , Zeisel S, Borchers C.H. , Ultra-high Field Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Studying the Human Metabolome, presented at the 53rd ASMS Conference on Mass Spectrometry and Allied Topics, June 2005, San Antonio, TX June 5-9, 2005 .
Hall M., Dokholyan N., Scarlett C.O., Taylor S., Petrotchenko E., Borchers C.H., Modeling the In Vivo Subunit Assembly of a Multi-Component Protein Complex by Discrete Molecular Dynamics using Data from a Binary In Vitro Interaction Study and Crosslinking combined with Mass Spectrometry. Presented at the Keystone Symposia for Proteomics and Bioinformatics, April 2005 Keystone, CO April 8-13 2005
Jiang J., Parker C.E. , Dicheva N., Fuller J.R., Kawula T.H., Borchers C.H.,A New Diagnostic Francisella Peptide Chip Assay Allowing Quantitative And Specific Detection Of F. tularensis presented at the 53rd ASMS Conference on Mass Spectrometry and Allied Topics, June 2005, San Antonio, TX June 5-9, 2005 .
Jiang J. , Warren E.N. , Parker C.E. , Dicheva N. , and Borchers C.H. ,MS-Based Protein Chip Technology For Rapid And Absolute Quantitation Of Expression And Modification Level For Cancer-Related Proteins, presented at the 53rd ASMS Conference on Mass Spectrometry and Allied Topics, June 2005, San Antonio, TX June 5-9, 2005 .
Petrotchenko E. and Borchers C.H.,Novel Isotopically-Coded Crosslinkers for Studying Protein-Protein Interactions in Proteomics. Presented at the Keystone Symposia for Proteomics and Bioinformatics, April 2005 Keystone, CO April 8-13 2005
Rouson, J., Kirksey, T., Warren, M.E.R., Borchers C.H.,
Does a Laboratory Information Managing System (LIMS) for Proteomics Laboratories need to be customized? Presented at the 53rd ASMS Conference on Mass Spectrometry and Allied Topics, June 2005, San Antonio, TX June 5-9, 2005 .
Scarlett C.O., Mocanu V., Jiang J., Lessey B.A., Dicheva N. , Parker C.E. , Borchers C.H., Plasma Cleanup: A Prerequisite for Biomarker Discovery, presented at the 53rd ASMS Conference on Mass Spectrometry and Allied Topics, June 2005, San Antonio, TX June 5-9, 2005 .
Torres M.P., Marzluff W., and Borchers C.H.,
Targeted Hypothesis-Driven Mass Spectrometry (THD-MS): A Synthesis of Methods for the Detection and Identification of Phosphopeptides. Presented at the Keystone Symposia for Proteomics and Bioinformatics, April 2005 Keystone, CO April 8-13 2005.
Warren E.N., Borchers C.H. ,
An MS-Based Immunoaffinity Approach For Quantitaion Of Protein Expression And Modification Levels. Presented at the Keystone Symposia for Proteomics and Bioinformatics, April 2005 Keystone, CO April 8-13 2005
Warren M.E.R, Loiselle D., Thelin W., Parker C.E., Dicheva N., Kesner B., Mocanu V., Wang F., Milgram S., Borchers C.H., In-gel Digestion from Unstained Gels for Increased Confidence in Protein Identification. Presented at the Keystone Symposia for Proteomics and Bioinformatics, April 2005 Keystone, CO April 8-13 2005
2004
Warren, M.E.R. , Wonsetler, R., Loiselle, D., Dicheva, N. , Ostrowski, L., Floyd, C., and Borchers, C.H. Cellular Localization and Identification of Membrane & Intracellular Ciliary Proteins by Specific Enrichment, Differential Gel Electrophoresis, and Mass Spectrometry, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville, TN, May 23 - 27, 2004.
Petrotchenko, E.V., Sun, J., Dicheva, N. N., Stamler, J. S., Meissner, G., Borchers, C. H. Identification of free cysteines in the calcium release channel, using fluorescent labeling and mass spectrometry, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville, TN, May 23 - 27, 2004.
Speir, P.S., Mocanu, V., Dominski, Z., Marzluff, W.F., Borchers, C.H. Simultaneous De-novo Sequencing and Determination of Modification Status and Modification Sites by Top-down Proteomics, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville, TN, May 23 - 27, 2004.
Warren, E. N., Elms, P. J., Parker, C. E., Merrick . B. A. , Wang, F., Pearson, R. E., Borchers, C. H. A Novel Mass Spectrometric Protein Chip for Absolute Quantitation of Protein Expression and Modification Levels, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville, TN , May 23 - 27, 2004.
Scarlett, C. O., Borchers, C.H. , Optimization of an LC-MALDI Interface for the Analysis of Phosphopeptides, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville , TN , May 23 - 27, 2004.
Torres, M.P., Borchers, C.H. Rapid Detection and Unambiguous Identification of Phosphopeptides from Complex Mixtures using On-Target Phosphatase Assays Combined with IMAC and MALDI MS/MS, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville , TN , May 23 - 27, 2004 .
Warren , M.E.R., Parker, C.E., Mocanu, V. , Klapper, D.G., Borchers, C.H. Fragmentation of Ubiquitinated Peptides: Optimizing Conditions for Generating Novel Ubiquitin-Specific Fragment Ions, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville , TN , May 23 - 27, 2004 .
Hall, M.C., Warren , E.N., Dial, J.M., Borchers, C.H. An LC-MALDI method for the efficient identification of in vivo phosphorylation sites on a TOF/TOF mass spectrometer, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville , TN , May 23 - 27, 2004 .
Loiselle, D. R., Dicheva, N. N ., Wang, F.,Thelin, W., Milgram, S., Borchers, C.H. Improved Protein Identification through the Use of Unstained Gels, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville , TN , May 23 - 27, 2004 .
Petrotchenko, E.V., Olkhovik, V. K., Borchers, C. H. A New Isotopically-labeled Cleavable Cross-Linker for Studying Complex Protein Assemblies, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville , TN , May 23 - 27, 2004 .
Mocanu, V. , Graham, L. B., Neamati, N., Solouki, T., Petrotchenko, E. V., Borchers, C. H. Top-down and Bottom-up Mass spectrometry Combined with Photoaffinity Labeling for Structural Characterization of Drug Binding Sites in HIV-Integrase, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville, TN, May 23 - 27, 2004 .
Petrotchenko, E.V., Wonsetler, R.L., Parker, C.E., Loiselle, D, R., Dicheva, N. N., Ostrowski, L. E., Borchers, C. H. Membrane Proteomics: Mass Spectrometric Identification Of Membrane Proteins Using Biotinylation And Affinity Purification, presented at the 52nd ASMS Conference on Mass Spectrometry and Allied Topics, May 2004, Nashville, TN, May 23 - 27, 2004 .
Borchers, C.H. , Bottom-up and Top-down Proteomics for Comprehensive Protein Characterization , presented at the 17th Annual Conference on Tandem Mass Spectrometry, Lake Louise , AB , Canada , Dec. 1-5, 2004 .
2003
Thorp, S., Chen, J. 1, Carrrick, K.L., Liu, J., Pope, R.M. Using MS approaches to study heparan sulfate and its biosynthesis, presented at the 51st Annual Conference on Mass Spectrometry and Allied Topics, Montreal, CAN, June 8-12, 2003.
Hwang, J.R., Parker, C.E., Jiang, J., Elms, P.J., Pope, R.M., Patterson, C., Borchers, C.H. Mass Spectrometric Elucidation of the in-vivo Mechanism of an E3 Ubiquitin Ligase, presented at the 51st Annual Conference on Mass Spectrometry and Allied Topics, Montreal, CAN, June 8-12, 2003.
Alekseev, O., Richardson, R.T., Loiselle, D., Parker, C., Borchers, C., Pope, M., O'Rand, M.G. MS Proteomic Method to Determine Binding Partners of NASP (Nuclear Autoantigenic Sperm Protein), presented at the 51st Annual Conference on Mass Spectrometry and Allied Topics, Montreal, CAN, June 8-12, 2003.
Cummins, T.D., Carrick, K., Loiselle, D., Pope, M., Borchers, C ., Collins, E.J. Use of Mass Spectrometry to Detect and Identify Francisella tularensis , a Potential Biological Weapon, presented at the 51st Annual Conference on Mass Spectrometry and Allied Topics, Montreal, CAN, June 8-12, 2003.
Wauson, E.M., Han, J., Carrick, K.L.,Pope, M., Graves, L.M. Characterization of Phospho-peptides in the Multifunctional Enzyme CAD, presented at the 51st Annual Conference on Mass Spectrometry and Allied Topics, Montreal, CAN, June 8-12, 2003.
Tie, J.K., Carrick, K.L., Mutucumarana, V.P., Lin, P.J., Scott, C., Wilcox, C.S., Pope, M., Stafford, D.W. Identification of active-site cysteines in Vitamin K-dependent gamma-glutamyl carboxylase by chemical modification, selected proteolysis, and MS/MS analysis, presented at the 51st Annual Conference on Mass Spectrometry and Allied Topics, Montreal, CAN, June 8-12, 2003.
Torres, M.P., Parker, C.E., Hall, M.C., Borchers, C.H. Identification and Monitoring of Cell-Cycle Dependent Dynamic Post-Translational Modifications in the 13-Subunit Anaphase Promoting Complex by Mass Spectrometry, presented at the 51st Annual Conference on Mass Spectrometry and Allied Topics, Montreal, CAN, June 8-12, 2003.
2002
Han, J., Pope, M., Borchers, C., Graves, L. Mapping of Protein Phosphorylation by Dual Enzyme Digestion and MALDI/qTOFMS, presented at the 50th Annual Conference on Mass Spectrometry and Allied Topics, Orlando, FL, June 2-6, 2002.
Raska, C.S., Parker, C.E., Pope, M., Huang, C., Han, J., Glish, G.L., Borchers, C.H. Pseudo-MS3 in a MALDI Orthogonal Injection Quadrupole Time-of Flight, presented at the 50th Annual Conference on Mass Spectrometry and Allied Topics, Orlando, FL, June 2-6, 2002.
Raska, C.S., Krause, M.C., Parker, C.E. , Dominski, Z., Marzluff, W.F., Glish, G.L., Pope, R.M., Borchers, C.H. Determination of the Phosphorylation Status and Phosphorylation Sites of the Stem Loop Binding Protein from Drosophila Melanogaster, presented at the 50th Annual Conference on Mass Spectrometry and Allied Topics, Orlando, FL, June 2-6, 2002 .
Kast, J., van der Drift, K., Dial, J.M., Borchers, C. Nano-electrospray MS/MS Analysis of Peptides Not Detected in the MS Mode, presented at the 50th Annual Conference on Mass Spectrometry and Allied Topics, Orlando , FL , June 2-6, 2002 .
Raska, C.S., Parker, C.E., Dominski, Z.,Marzluff, W.F., Sunnarborg, S.W., Lee, D.C., Glish, G.L., Pope, R.M., Borchers, C.H., Direct MALDI-MS/MS of Peptides Bound to Affinity Media, presented at the 50th Annual Conference on Mass Spectrometry and Allied Topics, Orlando, FL, June 2-6, 2002.
