Services Overview

Quicklink to Fees

Protein Identification (Core Service)

The proteomics facility offers 2 levels of protein identification services. All protein identification services include searches of publicly available databases and a results report. Database searches of client-provided sequences or specific modifications are available for an additional charge.

Identification of gel-separated proteins
Protein bands or spots separated by 1-D or 2-D SDS-PAGE are excised and digested in-gel (typically with trypsin, other enzymes available if needed upon consultation) and the resulting peptides are analyzed by ABI 4800 MALDI TOF/TOF MS. Charges include a setup fee for in-gel digestion and a fee per individual protein band or spot analyzed.

Identification of proteins or peptide mixtures
The facility also offers a larger scale anaylsis of proteins in solution derived from co-immunoprecipitation or similar experiments. Proteins of interst are digested in-solution and the resulting peptides are analyzed by ThermoScientific LTQ OrbiTrap LC-MS. Services include database searching and protein identification reports and simple C4 or C18 sample desalting.

Phosphorylation Analysis (Core Service)

The facility offers protein phosphorylation analysis of purified proteins or proteins derived from 1-D or 2-D SDS-PAGE. For proteins from SDS-PAGE an in-gel digestion setup fee applies. All other proteins are digested in-solution and peptides analyzed by ThermoScientific LTQ OrbiTrap LC-MS. Services include database search and reporting as well as simple C4 or C18 sample desalting.

Peptide/Protein Analysis (Core Service)

The proteomics facility offers mass analysis of purified peptides and proteins by ABI 4800 MALDI TOF MS. In addition, the facility offers peptide sequence analysis (peptides of known or unknown sequence) by ABI 4800 MALDI TOF/TOF MS. Service includes database search/sequence analysis and report.

Instrument Training

Training on ABI 4800 MALDI TOF/TOF

Services will include training in the preparation and analysis of proteins and peptides by ABI 4800 MALDI TOF/TOF mass spectrometry. Training will include basic principles of MALDI analysis (both MS and MS/MS), instrument calibration and usage, method set-up, sample analysis and data analysis. Once trained, the user will be able to calibrate the instrument, set up MS and MS/MS “jobs” and perform database searches (GPS Explorer/ Protein Pilot) to obtain the results of these analyses. Time: approximately 4 hrs total (split over more than one day).

Training on ThermoScientific LTQ-OrbiTrap

This training is for advanced users only and prior MALDI TOF/TOF training is required. Training will include sample preparation and instruction in instrument operations including calibration, software navigation, sample injection, LC maintenance and operations. The trained user will be able to prepare samples and will be able to successfully calibrate and set the instrument parameters to successfully analyze samples by MS and MS/MS. Time: 8 hrs total (split over multiple visits).

Mascot/Sequest Analysis

This will include additional training in the Mascot database and Sequest search algorithms.
Time: approximately 1 hr.

Fees

Services		Fees for UNC Investigators*
Protein Identification
	1. Gel/spot digestion set up fee	$200
	2. Protein Identification by MALDI-TOF/TOF
	• Per band or spot	$50
	• Approved trained-user fee (per hour)	$50
	3. Protein Identification by LTQ OrbiTrap
	• LTQ OrbiTrap (per run)	$200
	• Approved trained-user fee (per run)	$100
Phosphorylation Analysis
	1. Gel digestion set up fee	$200
	2. Phosphorylation analysis by OrbiTrap
	• Core Service LC-MS/MS on LTQ-OrbiTrap (per run)	$400
	• Approved trained-user fee (per sample run)	$200
Purified Peptide Analysis (Core Service)
	1. Peptide mass measurement	$20
	2. Peptide sequence analysis (w/known sequence)	$30
	3. Peptide sequence analysis (w/unknown sequence)	$100
Additional Data Analysis
	1. Additional data analysis (per hour)	$50
Instrument Training Fees
	1. Approved training on 4800 TOF/TOF	$300
	2. Approved training on LTQ-OrbiTrap	$500
	3. Approved training on Mascot/Sequest Analysis	$100

* The fees for non-UNC investigators are double the amount of the fees listed with the exception of the gel set up fee which is the same for either UNC or non-UNC investigators.